T4 ligase hc

Author: iegf

August undefined, 2024

http://www.enzymatics.com/pdf2/L6030-HC-L.pdf Web10 µL 2X Rapid Ligase Buffer X Vector (1 -10 ng/µL ) Y Insert (1 -10 ng/µL ) 1 µL T4 DNA Ligase ( 10 U/ µL) To 20 µL Nuclease -free Water . 2. Mix well by pipetting. 3. Incubate at 25 °C for 10 minutes 4. Purify DNA using a PCR clean-up column and elute in ~50 µL. – OR – Immediately dilute in TE or water (at least 1:10).

T4 DNA Ligase - Enzymatics

WebT4 RNA Ligase 1 Add to Cart: L6050L: 10,000 U: 20,000 U/ml: $443: Single-stranded RNA ligase. Also joins single-stranded DNA molecules. Add to Cart: T4 DNA Ligase (Rapid) Add to Cart: L6030-HC-L: 240,000 U: 600,000 U/ml: $707 “Ultrapure ligase” referenced in Nature Methods (Quail, 1 December 2008). Add to Cart: T3 DNA Ligase Add to Cart ... WebT4 DNA Ligase catalyzes the joining of two cohesive- or blunt-ended strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides. The enzyme will not join single-stranded nucleic acids. T4 DNA Ligase is provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl 2, 100mM DTT and 10mM ATP. tpinsure

Glycerol-Free T4 DNA Ligase (HC) Meridian Bioscience

WebLigation Protocol with T4 DNA Ligase (M0202) Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the … Web搜索到 25 条记录 . 分类. 突变 (1) 克隆试剂盒 WebT4 DNA Ligase (Rapid) Part Number L6030-HC-L Concentration 600,000 U/mL Unit Size 240,000 U Product Information Sheet L6030-HC-L Rev F Product Specification Storage … thermos colombia

Vaccines Free Full-Text Towards the Generation of an ASFV …

T4 DNA Ligase requires ATP as a cofactor. Highlights • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) • Fast—sticky-end ligation is completed in 10 minutes at room temperature • Supplied with PEG solution for efficient blunt-end ligation Applications WebCatalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single ... t pins lowe\u0027sWebT4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single … t pins knitting size

"WebJun 8, 2016 · High performance of T4 ligase is depending on integrity of the ATP in buffer. to keep the balance between T4 ligase optimal activity temperature (25 c) and ATP hydrolysis issue also cross... " - T4 ligase hc

T4 ligase hc

Thermo Scientific™ T4 DNA Ligase, HC (30 U/µL)

WebJun 10, 2013 · The T4 DNA ligase is the product of gene 30 ( lig) and it is essential for sealing Okazaki fragments during replication of the phage genome ( Miller et al., 2003 ). Consistent with its physiological role, in vitro it is highly efficient at sealing single-stranded nicks in duplex DNA. WebThe T4 DNA Ligase requires ATP as a cofactor. Highlights. Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) Fast – sticky …

Did you know?

Web560 3' -Terminal labelling of RN A with T 4 RN A ligase T4 RNA LIGASE catalyses the formation of an internucleotide phosphodiester bond between an oligonucleotide donor mole cule with a 5 ... WebT4 RNA Ligase 一本鎖RNAまたは一本鎖DNAの5'-末端のリン酸基と3'-末端の水酸基をATP依存的に結合 M1051 関連製品 T4 Polynucleotide Kinase DNAシークエンシング、DNA-タンパク質フットプリンティングのプローブとして一本鎖、二本鎖DNAやRNAの5’-末端標識 M4101, M4103 Alkaline Phosphatase, Calf Intestinal (CIAP) DNA、RNA、リボおよ …

WebB6030 (10X T4 DNA Ligase Buffer) 10X T4 DNA Ligase Buffer (B6030) 500mM Tris-HCI 100 mM MgCl 2 50 mM DTT 10 mM ATP pH 7.6 @ 25°C Unit Definition 1 unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X T4 DNA Ligase Buffer following a 30 minute incubation at … http://www.enzymatics.com/pdf/L603-HC-L.pdf

WebAug 28, 2010 · PCR amplification-purified cDNA was cloned into the pGEM-T easy vector (Promega) by T4 DNA ligase and amplified in Escherichia coli, JM109. Four positive colonies were selected and assessed using EcoRIdigestion of mini-prepared DNA. ... Chou HC, Sheu JC, Huang GT, Wang JT, Chen DS. Albumin messenger RNA is not specific … WebT4 DNA Ligase will ligate these substrates: dsDNA Nicked DNA/RNA Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl …

WebT4 DNA Ligase ( NEB #M0202) Is the enzyme of choice for the majority of recombinant DNA applications. It can be used for for cohesive ends (10 mins RT) and blunt ends (2 …

http://www.enzymatics.com/pdf2/L6030-LC-L.pdf tp-intWebT4 DNA Ligase requires ATP as a cofactor. Highlights • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP) • Fast—sticky … tpint032/intapp_newWebT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5ʹ phosphate and a 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins … thermos columbusWebDec 1, 2024 · Item T4 DNA Ligase (HC) Company Promega Price Supplier Page View Company Product Page Catalog Number M1794 Quantity 500u Concentration 10–20u/μl … tp institute berlinWebRNA Circularization using T4 RNA Ligase 1 Protocol for ssDNA/RNA Ligation (M0319) Ligation of a DNA or RNA oligo to single stranded RNA using T4 RNA Ligase 1 3´ End Labeling of RNA using T4 RNA Ligase 1 Typical Protocol for RNA Ligation (M0458) Featured Products T4 RNA Ligase 2, truncated K227Q T4 RNA Ligase 1 (ssRNA Ligase) tp interaction rho x et pakWebT4 DNA Ligase (HC), 500u. M1794 PDF (220 KB) – English (United States) thermos comidaWebSource of Protein: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene. Unit Definition: 1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C. Molecular weight: 55,292 Daltons. Quality Control ... tpinstop.exe