Top/fopflash reporter assay

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August undefined, 2024

Web86. Millipore luciferase top flash. Luciferase Top Flash, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebIn our lab, we usually transfect 50ng of DNA per well of a 96 well plate (in which we plate between 10-30K cells, depending cell line). From that 50ng DNA, 15ng are TOPFlash …

TOP/FOP-FLASH assay in HEK293T cells? ResearchGate

WebLuciferase reporter assays were performed by transfecting TOP/FOP Flash (A), Hes1 (B) or Hey1 (C) luciferase reporter in the cells treated with or without rapamycin (Rapa, 200nM, Sigma), an ... WebJournal: PLoS ONE Article Title: The natural compound Jatrophone interferes with Wnt/β-catenin signaling and inhibits proliferation and EMT in human triple-negative breast cancer doi: 10.1371/journal.pone.0189864 Article Snippet: The activity of β-catenin dependent Wnt signaling was measured by the TOPFLASH reporter using the Dual Luciferase Reporter … fasb 2016-02 leases

Luciferase Reporter Assay Reporter Gene Assay - YouTube

Web12. apr 2024 · The global Reporter Gene Assay market size is projected to grow from USUSD 1344.7 million in 2024 to USUSD 2010.3 million in 2029; it is expected to grow at a CAGR of 5.9Percent from 2024 to 2029 ... Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay was used to analyze the activity of Wnt/β-catenin signaling pathway. The vector pRL-SV40 was served as the internal reference. Cells in the control groups were co-transfected with pRL-SV40 and TOP/FOP flash (Promega). Cells in NC groups were co-transfected with pRL-SV40, TOP/FOP flash and si … WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0,... free tv and movies on the internet

Top Fop Flash Reporters Addgene inc Bioz

WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold … WebTOP/FOP-FLASH assay in HEK293T cells? Has anyone tried to study Wnt signaling in HEK293T cells? I have been trying to measure the Wnt activity using TOP/FOP-FLASH … free tv and movies online to watchWeb1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板，在其多克隆位点插 … free tv and movies online streaming sites

"WebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. … " - Top/fopflash reporter assay

Top/fopflash reporter assay

レポーター遺伝子アッセイ Thermo Fisher Scientific - JP

Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay and western blot were used to analysis the activation of Wnt/β-catenin signaling pathway. Si-CASC11-transfected HeLa cells were subcutaneously inoculated into male athymic (nude) mice to investigate the effect of CASC11 on the tumor formation. WebPred 1 dňom · A dual reporter assay is based on the simultaneous expression and measurement of two individual reporter enzymes within a single system. Typically, the primary or the experimental reporter is the marker of the gene or response element in question, whereas the activity of the co-transfected secondary or control reporter serves …

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WebPočet riadkov: 10 · TOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor …

Web6. apr 2024 · The TOP/FOP-FLASH assay showed that the canonical Wnt activities were reduced in HONE1 and NPC43 cells, when THY1 was over expressed (Figure 2A). ... Luciferase reporter HONE1 cells were injected into nude mice via tail vein, followed by treatment with 10 mg/kg of KX2-391 or solvent control at a frequency of twice per week … WebTop和Fop序列基本上一致的，Top的荧光素酶启动子上有几个Lef/TCF的结合位点，而在FOp是突变的，因此Top/Fop可以检测Wnt的活性改变。 1.应该是要用Top/FOP值来表示 …

WebDescription The TCF/LEF Reporter kit is designed for monitoring the activity of Wnt / β-catenin signaling pathway in the cultured cells. The kit contains transfection-ready … Web1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板，在其多克隆位点插 …

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Web王志国，饶艳伟(1.赤峰市松山医院骨科，内蒙古赤峰 024005；2.吉林省人民医院重症医学科，吉林长春 130021)骨肉瘤是一种常 free tv antennas walmartWeb26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a … free tv and streamingWeb10. jan 2024 · TOP/FOP-Flash reporter assay showed that CDX2 knockdown or CDX2 overexpression significantly increased or decreased Wnt signaling activity. Western blot assay showed that downstream targets... free tv antenna walmartWebTop Fop Flash Assay, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... Article Snippet: The TOP/FOP flash reporter plasmids containing wild-type (CCTTTGATC; TOP flash) or mutated (CCTTTGGCC; ... free tv antenna direction aimingWebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is … free tv antenna for all channelsWeb11. apr 2024 · We therefore performed a TOP/FOP luciferase reporter assay to monitor β-catenin transcriptional activity in the nucleus of SW620 and Difi cells. ... The TOP/FOP Flash luciferase assay shows the transcriptional activation of the Wnt/β-catenin signaling pathway in SW620 cells after oxaliplatin incubation (5µM for 72 h) compared with untreated ... free tv antenna in santa rosa beachWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : … free tv antenna project